Invitrogen

ADORA2A Monoclonal Antibody (6A6G11)

View all (17) ADORA2A antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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ADORA2A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ADORA2A in Hela cells (green). Sample was incubated with ADORA2A monoclonal antibody (Product # MA5-31611) using a dilution of 1:100-1:500 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

ADORA2A Antibody in Western Blot (WB) — Western blot analysis of ADORA2A in HEK293 (1) and ADORA2A cell lysate. Samples were incubated with ADORA2A monoclonal antibody (Product # MA5-31611) using a dilution of 1:500-1:2000.

ADORA2A Antibody in Flow Cytometry (Flow) — Flow cytometry of ADORA2A in HeLa cells (green). Sample was incubated with ADORA2A monoclonal antibody (Product # MA5-31611) using a dilution of 1:200-1:400 followed by negative control (red).

ADORA2A Antibody in ELISA (ELISA) — ELISA analysis of ADORA2A in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with ADORA2A monoclonal antibody (Product # MA5-31611) using a dilution of 1:10,000.

Product Details

MA5-31611

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Flow Cytometry (Flow)	1:200-1:400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	6A6G11
Immunogen	Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. coli. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2787235

Target Information

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.