Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product# MA3-060 ) shows staining in Hela Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing ADP-Ribosylation Factor (Product# MA3-060 ) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Non-human primate, Hamster, Bovine, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human ARF1.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/20|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.
MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome.
MA3-060 was used in immunocytochemistry and western blot to report that the BAR domain of ASAP1 is a protein binding site for FIP3
|Inoue H,Ha VL,Prekeris R,Randazzo PA||Molecular biology of the cell (19:4224)||2008|
PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal.
MA3-060 was used in immunocytochemistry to study the roles of phosphatidylinositol 4 phosphate in cargo recognition at the trans-Golgi network.
|Wang J,Sun HQ,Macia E,Kirchhausen T,Watson H,Bonifacino JS,Yin HL||Molecular biology of the cell (18:2646)||2007|
Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes.
MA3-060 was used in immunocytochemistry to investigate the role of G-protein-regulated PLA2 enzyme in membrane tubule formation and trafficking
|Chan D,Strang M,Judson B,Brown WJ||Molecular biology of the cell (15:1871)||2004|
Regulation of Golgi structure and function by ARF-like protein 1 (Arl1).
MA3-060 was used in immunocytochemistry to demonstrate the role of ARF-like protein 1 Arl1 in Golgi structure and function by ARF-like protein 1.
|Lu L,Horstmann H,Ng C,Hong W||Journal of cell science (114:4543)||2001|
ARF1 is directly involved in dynamin-independent endocytosis.
MA3-060 was used in western blot to investigate the important role of ARF1 in endocytosis
|Kumari S,Mayor S||Nature cell biology (10:30)||2008|
Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication.
MA3-060 was used in western blot to study the role of cellular Arf GTPases during poliovirus replication
|Belov GA,Habbersett C,Franco D,Ehrenfeld E||Journal of virology (81:9259)||2007|
Arf6 plays an early role in platelet activation by collagen and convulxin.
MA3-060 was used in western blot to study the role of Arf6 in platelet activation by collagen and convulxin
|Choi W,Karim ZA,Whiteheart SW||Blood (107:3145)||2006|
Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.
MA3-060 was used in western blot to investigate the role of ARF in poliovirus RNA replication
|Belov GA,Fogg MH,Ehrenfeld E||Journal of virology (79:7207)||2005|
Requirement for Arf6 in breast cancer invasive activities.
MA3-060 was used in western blot to investigate the role of ADP-ribosylation factor 6 (Arf6) in breast cancer invasive activities
|Hashimoto S,Onodera Y,Hashimoto A,Tanaka M,Hamaguchi M,Yamada A,Sabe H||Proceedings of the National Academy of Sciences of the United States of America (101:6647)||2004|
A role for calcium in stabilizing transport vesicle coats.
MA3-060 was used in western blot to study the role of calcium in transport vesicle coats stabilization.
|Ahluwalia JP,Topp JD,Weirather K,Zimmerman M,Stamnes M||The Journal of biological chemistry (276:34148)||2001|
An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization.
MA3-060 was used in western blot to investigate the role of ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 in paxillin subcellular localization and cytoskeletal organization
|Mazaki Y,Hashimoto S,Okawa K,Tsubouchi A,Nakamura K,Yagi R,Yano H,Kondo A,Iwamatsu A,Mizoguchi A,Sabe H||Molecular biology of the cell (12:645)||2001|
GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.
MA3-060 was used in western blot to demonstrate that GGAs are components of coats involved in protein trafficking at the TGN
|Dell'Angelica EC,Puertollano R,Mullins C,Aguilar RC,Vargas JD,Hartnell LM,Bonifacino JS||The Journal of cell biology (149:81)||2000|
ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes.
MA3-060 was used in western blot to investigate the role for ADP-ribosylation factor 1 in the membrane recruitment of AP-3 adaptor complex .
|Ooi CE,Dell'Angelica EC,Bonifacino JS||The Journal of cell biology (142:391)||1998|
|Non-human primate||Not Cited||
Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake.
MA3-060 was used in ELISA to investigate the role of the BIG2 in Tfn uptake and TfnR recycling.
|Shen X,Xu KF,Fan Q,Pacheco-Rodriguez G,Moss J,Vaughan M||Proceedings of the National Academy of Sciences of the United States of America (103:2635)||2006|
Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells.
MA3-060 was used in immunoprecipitation to demonstrate the role of ICA69 in membrane trafficking at the Golgi complex and immature secretory granules in neurosecretory cells.
|Spitzenberger F,Pietropaolo S,Verkade P,Habermann B,Lacas-Gervais S,Mziaut H,Pietropaolo M,Solimena M||The Journal of biological chemistry (278:26166)||2003|