Western blot analysis of ADP-ribose Polymerase 1 was performed using nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with etoposide (100uM Etoposide for 6hrs) (Lane 2) and HEK-293 (Lane 3). The blots were probed with Anti-ADP-ribose Polymerase 1 Mouse Monoclonal Antibody (Product # A21969,1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). An 85 kDa band corresponding to ADP-ribose Polymerase 1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||N-terminal end formed by the cleavage adjacent to Asp214|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 ug/ml|
|Immunocytochemistry (ICC)||1.0 ug/ml|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This PARP-1 (cleaved) antibody reacts with the N-terminal end of the apoptosis-specific 89 kDa catalytic domain fragment formed by the cleavage adjacent to Asp214. It does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.