Immunofluorescent analysis of ADP-ribosylation Factor 6 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ADP-ribosylation Factor 6 monoclonal antibody (Product # MA5-11795) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-11795 targets ADP-ribosylation Factor 6 in WB and ICC/IF applications and shows reactivity with Human and Mouse samples.
The MA5-11795 immunogen is aRF6 protein.
ARF6 (ADP-ribosylation factor 6) is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell-surface and intracellular membranes and whose functions may include tissue-specific effects on vesicular trafficking during cellular differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of nonapoptotic cell death by activated Ras requires inverse regulation of Rac1 and Arf6.
MA5-11795 was used in western blot to investigate the role of Ras, Rac1 and Arf6 in methuosis
|Bhanot H,Young AM,Overmeyer JH,Maltese WA||Molecular cancer research : MCR (8:1358)||2010|