Proteintech

ADRBK1 Monoclonal Antibody (2F7A5)

View all (27) GRK2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)

ADRBK1 Antibody in Immunocytochemistry (ICC/IF)

ADRBK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

ADRBK1 Antibody (67067-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ADRBK1 Antibody in Immunocytochemistry (ICC/IF)

ADRBK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADRBK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADRBK1 Antibody in Western Blot (WB)

Product Image Gallery

ADRBK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ADRBK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADRBK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADRBK1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Product Details

67067-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunocytochemistry (ICC/IF)

1:400

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2F7A5

Immunogen

ADRBK1 Fusion Protein Ag5071

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Phytotherapy research : PTR

Jiang M,Tang C,Yang M,Li Y,Wang W,Wang C,Wei W,Chen J

Wed Feb 01 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: adrenergic beta receptor kinase 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; EC 2.7.1.126; G-protein coupled receptor kinase 2; grk 2

View more View less

Gene Aliases: ADRBK1; BARK; BARK1; BETA-ARK1; GRK2

View more View less

UniProt ID: (Human) P25098

View more View less

Entrez Gene ID: (Human) 156

View more View less

protein kinase activity G-protein coupled receptor kinase activity protein binding ATP binding alpha-2A adrenergic receptor binding Edg-2 lysophosphatidic acid receptor binding beta-adrenergic receptor kinase activity non-receptor serine/threonine protein kinase

desensitization of G-protein coupled receptor protein signaling pathway negative regulation of the force of heart contraction by chemical signal G-protein coupled acetylcholine receptor signaling pathway tachykinin receptor signaling pathway heart development peptidyl-serine phosphorylation peptidyl-threonine phosphorylation viral genome replication receptor internalization positive regulation of catecholamine secretion negative regulation of striated muscle contraction viral entry into host cell cardiac muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support