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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:400 | - |
Immunocytochemistry (ICC/IF) |
1:400 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
2F7A5 |
Immunogen |
ADRBK1 Fusion Protein Ag5071 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1000 µg/mL |
Purification |
Protein A |
Storage buffer |
PBS with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Aliquoting is unnecessary for -20°C storage.
G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adrenergic beta receptor kinase 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; EC 2.7.1.126; G-protein coupled receptor kinase 2; grk 2
Gene Aliases: ADRBK1; BARK; BARK1; BETA-ARK1; GRK2
UniProt ID: (Human) P25098
Entrez Gene ID: (Human) 156
Molecular Function:
non-receptor serine/threonine protein kinase
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