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|Tested species reactivity||Human, Mouse, Primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant fragment of human AIF expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15880 targets AIF in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15880 immunogen is purified recombinant fragment of human AIF expressed in E. Coli. .
MA5-15880 detects AIF which has a predicted molecular weight of approximately 67kDa.
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
From ventriculomegaly to severe muscular atrophy: expansion of the clinical spectrum related to mutations in AIFM1.
MA5-15880 was used in western blot to discuss the known phenotypes related to AIFM1 mutations and further study a single patient
|Kettwig M,Schubach M,Zimmermann FA,Klinge L,Mayr JA,Biskup S,Sperl W,Gärtner J,Huppke P||Mitochondrion (21:12)||2015|
apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; harlequin; programmed cell death 8 (apoptosis-inducing factor); programmed cell death protein 8; RP3-438D16.2, AIF, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; testicular secretory protein Li 4
AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8