|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 1-50 of human AKAP 10|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 74 kDa.
Purity is >95% by SDS-PAGE.
AKAP 10 (A kinase anchor protein 10), also known as PRKA10 or D-AKAP2 (dual-specific A kinase-anchoring protein 2), is a 662 amino acid mitochondrial membrane protein that belongs to the AKAP family. AKAP 10 is a dual specificity protein that binds to both type I and type II regulatory subunits of PKA and anchors them to the plasma membrane or the mitochondria. When anchored to the mitochondria, PKA can phosphorylate and, thus, inactivate the proapoptotic protein Bad. This suggests that AKAP 10 indirectly regulates Bad-induced apoptosis by mediating the mitochondrial attachment of PKA. Additionally, AKAP 10 may facilitate G-protein coupled signal transduction and could act as an adaptor in the assembly of multiprotein complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.