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AKAP14 Antibody (MA5-38020) in WB
Western blot analysis of AKAP14 in extracts of various cell lines. Samples were incubated with AKAP14 Monoclonal antibody (Product # MA5-38020) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
MA5-38020
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2344
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 70-150 of human AKAP14 (Q86UN6)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897938
Product Specific Information
Positive Samples: A-549, DU145, Mouse testis, Rat testis
Immunogen sequence: HGEFTVEKGL KQIDEYFSKC VSKKCWAHGV EFVERKDLIH SFLYIYYVHW SISTADLPVA RISAGTYFTM KVSKTKPPDA P
Target Information
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein 14; A-kinase anchor protein 14; A-kinase anchor protein 28 kDa; A-kinase anchoring protein 28; AKAP 28; AKAP-14; PRKA14; Protein kinase A-anchoring protein 14; TAKAP-1.2; Testis-specific A-kinase-anchoring protein TAKAP-80; Testis-specific A-kinase-anchoring-protein
Gene Aliases: AKAP14; AKAP28; PRKA14; Takap80
UniProt ID: (Human) Q86UN5, (Rat) O35817
Entrez Gene ID: (Human) 158798, (Rat) 60332, (Mouse) 434756
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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