Immunofluorescent analysis of Akt (pan) in HeLa cells, insulin-treated, using an Akt (pan) monoclonal antibody (Product # MA5-14999) showing translocation of Akt from the cytoplasm to the membrane upon insulin treatment. Actin filaments have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide at the carboxy-terminal sequence of mouse Akt|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.