Invitrogen

AKT2 Polyclonal Antibody

Product Image Gallery

AKT2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of C2C12 cells stained with an AKT2 polyclonal antibody (Product # PA5-13760). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an AKT2 polyclonal antibody (Product # PA5-13760) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

AKT2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HeLa cells using an AKT2 polyclonal antibody (Product # PA5-13760) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

AKT2 Antibody in Western Blot (WB) — Western blot analysis of AKT2 (arrow) using an AKT2 polyclonal antibody (Product # PA5-13760) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).

AKT2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an AKT2 polyclonal antibody (Product # PA5-13760), followed by HRP-conjugated secondary antibody and DAB staining.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:10-1:50

Immunocytochemistry (ICC)

1:10-1:50

Immunofluorescence (IF)

1:10-1:50

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 416-444 amino acids from the C-terminal region of human AKT2

Conjugate

Unconjugated

Form

Liquid

Concentration

Lot-specific

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2242520

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AKT2; murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; Protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

Gene Aliases: 2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA

UniProt ID: (Human) P31751, (Mouse) Q60823

Entrez Gene ID: (Human) 208, (Mouse) 11652

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase