Invitrogen

AKT2 Polyclonal Antibody

View all (59) AKT2 antibodies

Questions & Answers

Questions & Answers

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AKT2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of C2C12 cells stained with an AKT2 polyclonal antibody (Product # PA5-13760). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an AKT2 polyclonal antibody (Product # PA5-13760) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

AKT2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an AKT2 polyclonal antibody (Product # PA5-13760), followed by HRP-conjugated secondary antibody and DAB staining.

AKT2 Antibody in Western Blot (WB) — Western blot analysis of AKT2 (arrow) using an AKT2 polyclonal antibody (Product # PA5-13760) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).

AKT2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HeLa cells using an AKT2 polyclonal antibody (Product # PA5-13760) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Product Details

PA5-13760

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Immunocytochemistry (ICC/IF)	1:10-1:50	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 416-444 amino acids from the C-terminal region of human AKT2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Lot-specific
Purification	Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2242520

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

Gene Aliases: 2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA

UniProt ID: (Human) P31751, (Mouse) Q60823

Entrez Gene ID: (Human) 208, (Mouse) 11652

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation cellular protein modification process apoptotic process response to osmotic stress signal transduction insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction negative regulation of plasma membrane long-chain fatty acid transport positive regulation of glucose metabolic process response to muscle activity peptidyl-serine phosphorylation regulation of cell migration positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance cellular response to insulin stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation intracellular signal transduction negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis mammary gland epithelial cell differentiation intracellular protein transmembrane transport regulation of cell cycle arrest cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus carbohydrate metabolic process glycogen metabolic process protein phosphorylation transport multicellular organism development positive regulation of gene expression positive regulation of sodium ion transport phosphorylation negative regulation of apoptotic process

Process(es)

protein serine/threonine kinase activity protein kinase C binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups