Invitrogen

ALDH6A1 Monoclonal Antibody (6H9B7)

Product Image Gallery

ALDH6A1 Antibody in Western Blot (WB) — Western blot analysis of ALDH6A1 in HEK293 (1) and ALDH6A1 cell lysate. Samples were incubated with ALDH6A1 monoclonal antibody (Product # MA5-31617) using a dilution of 1:500-1:2000.

ALDH6A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ALDH6A1 in paraffin-embedded ovarian cancer tissue. Sample was incubated with ALDH6A1 monoclonal antibody (Product # MA5-31617) using a dilution of 1:200-1:1000 followed by DAB staining.

ALDH6A1 Antibody in Flow Cytometry (Flow) — Flow cytometry of ALDH6A1 in HeLa cells (green). Sample was incubated with ALDH6A1 monoclonal antibody (Product # MA5-31617) using a dilution of 1:200-1:400 followed by negative control (red).

ALDH6A1 Antibody in ELISA (ELISA) — ELISA analysis of ALDH6A1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with ALDH6A1 monoclonal antibody (Product # MA5-31617) using a dilution of 1:10,000.

ALDH6A1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ALDH6A1 in Hela cells (green). Sample was incubated with ALDH6A1 monoclonal antibody (Product # MA5-31617) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Immunocytochemistry (ICC/IF)

1:200-1:1000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6H9B7

Immunogen

Purified recombinant fragment of human ALDH6A1 (AA: 1-195) expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787241

Target Information

This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aldehyde dehydrogenase 6 family, member A1; Aldehyde dehydrogenase family 6 member A1; malonate-semialdehyde dehydrogenase; Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; MGC40271; mitochondrial acylating methylmalonate-semialdehyde dehydrogenase; MMSDH; testicular tissue protein Li 122

Gene Aliases: ALDH6A1; MMSADHA; MMSDH

UniProt ID: (Human) Q02252

Entrez Gene ID: (Human) 4329

Molecular Function: dehydrogenase oxidoreductase