OriGene

ALG2 Monoclonal Antibody (OTI3C2), TrueMAB™

Product Image Gallery

ALG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

ALG2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2. Positive lysates LY409737 (100 µg) and LC409737 (20 µg) can be purchased separately from OriGene.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500-1:2000

Product Specifications

Species

Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3C2

Immunogen

Full length human recombinant protein of human ALG2 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1, 3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1, 3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha-1,3-mannosyltransferase ALG2; Alpha-1,3/1,6-mannosyltransferase ALG2; asparagine-linked glycosylation 2 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase); asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase); asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog; Asparagine-linked glycosylation protein 2 homolog; FLJ14511; GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase; GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; homolog of yeast ALG2

Gene Aliases: ALG2; CDGIi; CMS14; CMSTA3; hALPG2; NET38; UNQ666/PRO1298

UniProt ID: (Human) Q9H553

Entrez Gene ID: (Human) 85365

Molecular Function: carbohydrate phosphatase glycosyltransferase hydrolase nucleotidyltransferase phosphatase transferase