Immunofluorescent analysis of ALK (Anaplastic Lymphoma Kinase)/p80 (green) showing staining in the cytoplasm of NCI-H1299 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ALK/p80 monoclonal antibody (Product # MA5-14528) in 3% BSA-PBS at a dilution of 1:10 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-14528 targets ALK (Anaplastic Lymphoma Kinase) / p80 in IHC (P) and ICC/IF applications and shows reactivity with Human and Mouse samples.
The MA5-14528 immunogen is recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in 30-50% of CD30+ large cell lymphomas (1). This rabbit monoclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein (2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High anaplastic lymphoma kinase immunohistochemical staining in neuroblastoma and ganglioneuroblastoma is an independent predictor of poor outcome.
MA5-14528 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of ALK in patients with neuroblastoma or ganglioblastoma
|Duijkers FA,Gaal J,Meijerink JP,Admiraal P,Pieters R,de Krijger RR,van Noesel MM||The American journal of pathology (180:1223)||2012|
Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
MA5-14528 was used in immunohistochemistry to investigate the role of ALK overexpression in B-cell lymphoma/leukemia progression
|Giuriato S,Foisseau M,Dejean E,Felsher DW,Al Saati T,Demur C,Ragab A,Kruczynski A,Schiff C,Delsol G,Meggetto F||Blood (115:4061)||2010|
Diagnostic and differential diagnostic criteria of lymphoid neoplasms in bone marrow trephine biopsies: a study of 87 cases.
MA5-14528 was used in immunohistochemistry to investigate the diagnosis of lymphoid neoplasmas through bone marrow trephine biopsies
|Horváth E,Mezei T,Pávai Z,Turcu M,Demian S,Tóth E,Chira L,Jung I||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (50:399)||2009|
Development of a conditional bioluminescent transplant model for TPM3-ALK-induced tumorigenesis as a tool to validate ALK-dependent cancer targeted therapy.
MA5-14528 was used in immunohistochemistry to investigate the usefulness of a specific cancer model for the analysis of ALK-dependent therapy
|Giuriato S,Faumont N,Bousquet E,Foisseau M,Bibonne A,Moreau M,Al Saati T,Felsher DW,Delsol G,Meggetto F||Cancer biology and therapy (6:1318)||2007|