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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is anaplastic lymphoma.
This antibody recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High anaplastic lymphoma kinase immunohistochemical staining in neuroblastoma and ganglioneuroblastoma is an independent predictor of poor outcome.
MA5-16320 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of ALK in patients with neuroblastoma or ganglioblastoma
|Duijkers FA,Gaal J,Meijerink JP,Admiraal P,Pieters R,de Krijger RR,van Noesel MM||The American journal of pathology (180:1223)||2012|
ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD246; CD246 antigen; mutant anaplastic lymphoma kinase; NBLST3; TFG/ALK
ALK; CD246; NBLST3