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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Human, Rat |
Host/Isotype |
Mouse / IgG2b |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI3G8 |
Immunogen |
Full length human recombinant protein of human ALOX15 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 12-LOX; 12/15-lipoxygenase; 12/15-LOX; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; Polyunsaturated fatty acid lipoxygenase ALOX15
Gene Aliases: 12-LOX; 15-LOX; 15-LOX-1; 15LOX-1; Alox12; Alox12l; ALOX15; LOG15
UniProt ID: (Human) P16050, (Rat) Q02759
Entrez Gene ID: (Human) 246, (Dog) 489458, (Rat) 81639
Molecular Function:
oxygenase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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