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Immunofluorescent analysis of ALY (red) in HeLa cells. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 5-10 minutes at room temperature and washed 3 times with PBS containing 1% Fetal Calf Serum (wash buffer) at room temperature. Cells were probed with an ALY monoclonal antibody (Product # MA1-26754) at a dilution of 1:500 for 1 hour at room temperature, washed with wash buffer, and incubated with a fluorescently-conjugated anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on an LSM780 (Zeiss Microscope) at 63x magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human ALY / REF fusion protein.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1:500 - 1:1000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-26754 detects Aly from human and Xenopus laevis samples.
MA1-26754 has been successfully used in immunoprecipitation, immunofluorescence, ELISA, and Western blot procedures.
The MA1-26754 immunogen is recombinant human ALY / REF fusion protein.
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
MA1-26754 was used in western blot to elucidate the roles of nuclear cap-binding protein 1-3 in mRNA biogenesis
|Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A||Nature communications (6:null)||2015|
Ally of AML-1 and LEF-1; ALY; Aly/REF export factor; Aly/REF export factor-A; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; THO complex 4; THO complex subunit 4; THO complex subunit 4-A; Tho4; Tho4-A; Transcriptional coactivator Aly/REF
ALY; ALY/REF; ALYREF; alyref-a; BEF; REF; Tho4-A; THOC4; thoc4-a