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AMIGO1 Antibody (MA5-45620) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with AMIGO-1 monoclonal antibody (Product # MA5-45620) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red ... View More
Product Details
MA5-45620
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
L86/33
Immunogen
Fusion proteinamino acids 394-492 (cytoplasmic C-terminus) of mouse AMIGO-1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932074
Product Specific Information
1 µg/mL of MA5-45620 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 60-80kDa depending on maturity/glycosylation.
This antibody was formerly sold as clone S86-36.
Target Information
Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adhesion molecule with Ig-like domain 1; alivin 2; Alivin-2; AMIGO 1; AMIGO-1; AMIGO1; amigo1 {ECO:0000250|UniProtKB:Q80ZD8}; amphoretin-induced gene and ORF; amphoterin induced gene and ORF (Amigo); amphoterin-induced gene and ORF; Amphoterin-induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6; transmembrane protein AMIGO
Gene Aliases: ALI2; AMIGO; AMIGO-1; AMIGO1; KIAA1163
UniProt ID: (Human) Q86WK6, (Mouse) Q80ZD8, (Rat) Q80ZD7
Entrez Gene ID: (Human) 57463, (Mouse) 229715, (Rat) 295365
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
multicellular organism development
nervous system development
axonogenesis
axonal fasciculation
brain development
cell differentiation
myelination
positive regulation of axonogenesis
positive regulation of synapse assembly
cell-cell adhesion
positive regulation of potassium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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