OriGene

ANXA1 Monoclonal Antibody (OTI3A8), TrueMAB™

View all (28) Annexin A1 antibodies

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ANXA1 Antibody in Immunofluorescence (IF) — Anti-ANXA1 mouse monoclonal antibody (TA500967) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA1(RC201569).

ANXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

ANXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

ANXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

ANXA1 Antibody in Western Blot (WB) — Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO,Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control. (1:500)

ANXA1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA1 (RC201569, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA1. Positive lysates LY424560 (100 µg) and LC424560 (20 µg) can be purchased separately from OriGene.

ANXA1 Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-ANXA1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100

-

Immunofluorescence (IF)

1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Western Blot (WB)

1:2000

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3A8

Immunogen

Full length human recombinant protein of human ANXA1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Concentration

0.9 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; p35; Phospholipase A2 inhibitory protein; RP11-71A24.1

Gene Aliases: ANX1; ANXA1; LPC1

UniProt ID: (Human) P04083

Entrez Gene ID: (Human) 301

DNA strand renaturation neutrophil homeostasis adaptive immune response monocyte chemotaxis regulation of leukocyte migration phagocytosis inflammatory response signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger regulation of cell shape response to X-ray myoblast migration involved in skeletal muscle regeneration peptide cross-linking insulin secretion keratinocyte differentiation prostate gland development endocrine pancreas development positive regulation of vesicle fusion positive regulation of prostaglandin biosynthetic process actin cytoskeleton reorganization response to estradiol DNA duplex unwinding regulation of interleukin-1 production positive regulation of interleukin-2 production positive regulation of neutrophil apoptotic process DNA rewinding gliogenesis positive regulation of T cell proliferation response to drug negative regulation of apoptotic process response to peptide hormone estrous cycle innate immune response positive regulation of T-helper 1 cell differentiation negative regulation of T-helper 2 cell differentiation negative regulation of exocytosis alpha-beta T cell differentiation regulation of hormone secretion arachidonic acid secretion regulation of inflammatory response cellular response to hydrogen peroxide hepatocyte differentiation prolactin secretion response to interleukin-1 cellular response to glucocorticoid stimulus granulocyte chemotaxis positive regulation of wound healing neutrophil clearance positive regulation of G1/S transition of mitotic cell cycle negative regulation of phospholipase A2 activity negative regulation of interleukin-8 secretion

Process(es)

single-stranded DNA binding single-stranded RNA binding helicase activity receptor binding structural molecule activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding phospholipase A2 inhibitor activity protein binding, bridging double-stranded DNA-dependent ATPase activity annealing helicase activity protein homodimerization activity calcium-dependent protein binding cadherin binding involved in cell-cell adhesion