Western blot analysis of ANXA3/Annexin III using A) 30 µg A431 whole cell lysate (B) 30 µg HeLa whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ANXA3/Annexin III polyclonal antibody (Product # PA5-34829) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 45 and 272 of Annexin III (Uniprot ID#P12429)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34829 targets ANXA3/Annexin III in WB applications and shows reactivity with Human samples.
The PA5-34829 immunogen is recombinant fragment corresponding to a region within amino acids 45 and 272 of Annexin III (Uniprot ID#P12429).
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.