|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues I(5) R L R E L I R T I R T A R T Q A E E R(24) of human AP-1 complex subunit gamma-1.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:2000|
|Western Blot (WB)||1:1,000 - 1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18306 detects AP-1 complex subunit gamma-1 from human and rat samples.
PA1-18306 has been successfully used in immunohistochemistry (paraffin and frozen) and Western blot procedures.
The PA1-18306 immunogen is a synthetic peptide corresponding to the N-terminal residues I(5) R L R E L I R T I R T A R T Q A E E R(24) of human AP-1 complex subunit gamma-1, conjugated to diphtheria toxin. The sequence is 100% conserved in mouse, rat, dog and zebra fish.
Reconstitute with 100 ul of distilled water.
AP-1 complex subunit gamma-1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.