Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Chicken, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||N-terminus of human AP2a.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:500|
|Immunoprecipitation (IP)||1:100 - 1:250|
|Western Blot (WB)||1:50 - 1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-872 detects AP-2 from human, mouse, and chicken samples.
MA1-872 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence applications. By Western blot, this antibody detects a 45-50 kDa band representing AP-2. For Western blotting, block with BSA only (not milk).
The MA1-872 immunogen is the N-terminus of human AP2a.
AP-2, also known as ERF-1, plays an important role in regulating estrogen receptor expression. It also may play a role in the development of ectodermal-derived tissue. Mutations in the AP-2 gene have been linked to many eye chamber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.