Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Cell death signals are transduced by death domain containing adaptor molecules and members of the caspase family of proteases. The human and mouse homologues to C. elegans death receptor 4 (CED-4) have recently been isolated and named Apaf-1. Apaf-1 binds to cytochrome C and caspase-9 (also known as Apaf-2 and Apaf-3, respectively), leading to activation of caspase-9. Activated caspase-9 activates caspase-3, leading to the proteolytic cleavage of a number of different proteins involved in the apoptotic cascade. Apaf-1 is ubiquitously expressed in human tissues and has also been shown to interact with caspase-4 and caspase-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APAF-1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; forebrain overgrowth
Gene Aliases: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413