|Tested species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the human Apg12 protein. This sequence differs from the mouse sequence by one amino acid.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins.
36-6400 was used in western blot to investigate how hGBPs regulate the antichlamydial effect of IFNG.
|Al-Zeer MA,Al-Younes HM,Lauster D,Abu Lubad M,Meyer TF||Autophagy (9:50)||2013|
Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12¿5-16L2 complex.
36-6400 was used in immunocytochemistry to elucidate the function and regulation of the Atg12-5-16L1 complex.
|Ishibashi K,Fujita N,Kanno E,Omori H,Yoshimori T,Itoh T,Fukuda M||Autophagy (7:1500)||2011|
|Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein.||Sarkar S,Davies JE,Huang Z,Tunnacliffe A,Rubinsztein DC||The Journal of biological chemistry (282:5641)||2007|