|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (55 KLQAEKHDVPNRHEISPG 72) of APG16L protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1.0-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The process is found in eukaryotes, including yeast, and involves several genes and two novel ubiquitin-like conjugation systems. The APG12 system is one of the ubiquitin-like protein conjugation systems essential for autophagy. Covalent attachment of APG12–APG5 is essential for autophagy. In mammalian cells, the APG12–APG5 conjugate is a protein complex of approximately 800 kDa and includes an additional novel protein. This novel protein was designated APG16L (APG16-like, also referred to as ATG16L) as it's N-terminal region contains several features similar to yeast APG16. APG16L protein has several spliced isoforms and is larger than yeast APG16 (17 kD). The protein has a large C-terminal domain containing seven WD repeats. Genetic variations in the protein are associated with susceptibility to inflammatory bowel disease type 10. The human APG16L gene is localized in the chromosomal region 2q37.1.
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