|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues of human ATG3.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18297 detects ATG3 from human and rat samples.
PA1-18297 has been successfully used in immunohistochemistry, immunofluorescence, and Western blot procedures.
The PA1-18297 immunogen is a synthetic peptide corresponding to the N-terminal residues of human ATG3, conjugated to Blue Carrier Protein. The sequence is 100% conserved in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and canine.
Reconstitute with 100 ul of distilled water.
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.