|Tested species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (89 CRHLGRDWRWTQRKRQPDSYFS 110) of APG4B protein.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AG4B is a 393 amino acid cysteine protease consisting of the active catalytic triad site of Cys74, His280 and Asp278 and belonging to the peptidase C54 family. It is required for autophagy, which is essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death and aging. It negatively regulates the localization of LC3 to a membrane compartment by delipidation and mediates murine Atg8L/Apg8L modification during the formation of autophagosomes APG4B is expressed in skeletal muscle, brain, heart, liver and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.