|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues of human APG-5L.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18282 detects APG5 from human samples.
PA1-18282 has been successfully used in immunohistochemistry, immunofluorescence, and Western blot procedures.
The PA1-18282 immunogen is a synthetic peptide corresponding to the C-terminal residues of human APG-5L.
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. avoid repetitive freeze/thaw cycles.
Reconstitute with 100 ul of distilled water.
APG5 is required for autophagy. APG5 conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.