Immunofluorescent analysis of APG5 showing staining in the autophagosome of HepG2 cells. HepG2 cells treated with 3µM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min and stained using an APG5 polyclonal antibody (Product # PA5-29694) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 64 and 275 of Human APG5|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29694 targets APG5 in WB applications and shows reactivity with Human samples.
The PA5-29694 immunogen is recombinant fragment corresponding to a region within amino acids 64 and 275 of Human APG5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.