|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues of human APG7.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18299 detects ATG7 from human and rat samples.
PA1-18299 has been successfully used in immunohistochemistry, western blot, ELISA and immunofluorescence procedures.
The PA1-18299 immunogen is a synthetic peptide corresponding to the C-terminal residues of human APG7, conjugated to blue carrier protein. The sequence is 100% conserved in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and canine.
Reconstitute with 100 ul of distilled water.
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.