|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (554 NDVVAPGDSTRDRTLDQQC 572) of APG7L protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. A member of the autophagy family of proteins is APG7L which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7L is essential for autophagy conjugation systems, autophagosome formation, and starvation-induced bulk degradation of proteins and organelles. The protein thus plays an indispensable role in the initial step of the conjugation system. APG7L interacts with Apg8p/Aut7p and Aut1p/Apg3p in addition to Apg12p. The protein maps to the 3p25.3-p25.2 region of the human chromosome. Ablation of the protein leads to abnormal swellings and dystrophy of purkinje cell axon terminals in the deep cerebellar nuclei.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.