Western blot analysis of APOA4 using a APOA4 monoclonal antibody (Product # MA5-15485) against a truncated APOA4-His recombinant protein (1), truncated APOA4 (aa21-396) human IgG Fc transfected CHO-K1 cell lysate (2), human serum (3) and human plasma (4).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of APOA4 (aa21-396) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15485 targets APOA4 in WB applications and shows reactivity with Human samples.
The MA5-15485 immunogen is purified recombinant fragment of APOA4 (aa21-396) expressed in E. Coli.
Apoliprotein A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.