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Invitrogen
APOE Monoclonal Antibody (4E4)
FIGURE: 1 / 3
APOE Antibody (MA5-16146) in ICC/IF
Immunofluorescence analysis of APOE was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APOE Mouse Monoclonal Antibody (Product # MA5-16146) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at ... View More
Product Details
MA5-16146
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4E4
Immunogen
Synthetic peptide made to an internal region of human ApoE4 (within residues 100-150).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11157884
Target Information
Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571
Gene Aliases: AD2; APO-E; APOE; LDLCQ5; LPG
UniProt ID: (Human) P02649
Entrez Gene ID: (Human) 348
beta-amyloid binding
lipid transporter activity
protein binding
phospholipid binding
heparin binding
lipid binding
cholesterol binding
antioxidant activity
cholesterol transporter activity
identical protein binding
protein homodimerization activity
metal chelating activity
tau protein binding
low-density lipoprotein particle receptor binding
phosphatidylcholine-sterol O-acyltransferase activator activity
very-low-density lipoprotein particle receptor binding
lipoprotein particle binding
apolipoprotein
response to reactive oxygen species
retinoid metabolic process
negative regulation of endothelial cell proliferation
response to dietary excess
triglyceride metabolic process
cholesterol catabolic process
cellular calcium ion homeostasis
receptor-mediated endocytosis
cytoskeleton organization
G-protein coupled receptor signaling pathway
nitric oxide mediated signal transduction
synaptic transmission, cholinergic
cholesterol metabolic process
regulation of gene expression
negative regulation of platelet activation
positive regulation of cholesterol esterification
positive regulation of cholesterol efflux
long-chain fatty acid transport
protein import
virion assembly
triglyceride catabolic process
cGMP-mediated signaling
negative regulation of blood coagulation
regulation of axon extension
positive regulation of cGMP biosynthetic process
neuron projection regeneration
regulation of Cdc42 protein signal transduction
positive regulation of low-density lipoprotein particle receptor catabolic process
cholesterol efflux
phospholipid efflux
very-low-density lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
chylomicron remnant clearance
high-density lipoprotein particle clearance
very-low-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein biosynthetic process
lipoprotein catabolic process
vasodilation
cholesterol homeostasis
negative regulation of MAP kinase activity
negative regulation of neuron apoptotic process
negative regulation of blood vessel endothelial cell migration
reverse cholesterol transport
positive regulation by host of viral process
negative regulation of cholesterol biosynthetic process
positive regulation of lipid biosynthetic process
intracellular transport
regulation of neuronal synaptic plasticity
artery morphogenesis
negative regulation of inflammatory response
positive regulation of nitric-oxide synthase activity
positive regulation of membrane protein ectodomain proteolysis
maintenance of location in cell
fatty acid homeostasis
positive regulation of dendritic spine development
negative regulation of canonical Wnt signaling pathway
AMPA glutamate receptor clustering
NMDA glutamate receptor clustering
cellular oxidant detoxification
regulation of beta-amyloid clearance
negative regulation of neuron death
positive regulation of postsynaptic membrane organization
negative regulation of presynaptic membrane organization
negative regulation of beta-amyloid formation
positive regulation of dendritic spine maintenance
positive regulation of phospholipid efflux
positive regulation of lipid transport across blood brain barrier
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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