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Immunofluorescent colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and MA1-19728 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1; centaurin, delta 2; centaurin-delta-2; CENTD2; cnt-d2; KIAA0782
ARAP1; CENTD2; KIAA0782