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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Daudi cell lysate.
PA5-20374 can be used with blocking peptide PEP-0491.
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ARF binding protein 1; ARF-binding protein 1; ARF-BP1; BJ-HCC-24 tumor antigen; E3 ubiquitin-protein ligase HUWE1; E3Histone; HECT domain protein LASU1; HECT, UBA and WWE domain-containing protein 1; HectH9; Homologous to E6AP carboxyl terminus homologous protein 9; Large structure of UREB1; LASU1; Mcl-1 ubiquitin ligase; Mcl-1 ubiquitin ligase E3; Mule; RP3-339A18.4, ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, UREB1; upstream regulatory element binding protein 1; Upstream regulatory element-binding protein 1; URE-B1; URE-binding protein 1
5430439H10Rik; ARF-BP1; AU041296; C430014N20Rik; C80292; Gm1718; HECTH9; HSPC272; HUWE1; Ib772; KIAA0312; KIAA1578; LASU1; MULE; URE-B1; UREB1