This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.
MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein
Gene Aliases: 5430400P17Rik; AA407803; Ac1-253; AI788669; AI854770; ARF1; ARF2; ARF3; ARF4; ARF5; ARF6; AW496366; I79_003112
UniProt ID: (Human) P84077, (Human) P61204, (Human) P18085, (Human) P84085, (Human) P62330, (Mouse) P84078, (Rat) P84079, (Rat) P84082, (Mouse) P10947, (Mouse) P61205, (Rat) P61206, (Rat) P61751, (Mouse) P61750, (Mouse) P84084, (Rat) P84083, (Mouse) P62331, (Rat) P62332
Entrez Gene ID: (Dog) 100856517, (Human) 375, (Dog) 486562, (Human) 377, (Dog) 607073, (Human) 378, (Dog) 475206, (Human) 381, (Human) 382, (Dog) 490679, (Chinese hamster) 100757864, (Mouse) 11840, (Rat) 64310, (Rat) 79119, (Mouse) 11841, (Mouse) 11842, (Chinese hamster) 100766631, (Rat) 140940, (Chinese hamster) 100769922, (Rat) 79120, (Mouse) 11843, (Mouse) 11844, (Chinese hamster) 100759676, (Rat) 79117, (Mouse) 11845, (Chinese hamster) 100774654, (Rat) 79121
Molecular Function: G-protein