This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.
MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.
ARF4 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein
Gene Aliases: 5430400P17Rik; AA407803; Ac1-253; AI788669; AI854770; ARF1; ARF2; ARF3; ARF4; ARF5; ARF6; AW496366; I79_003112
UniProt ID: (Human) P84077, (Human) P61204, (Human) P18085, (Human) P84085, (Human) P62330, (Mouse) P84078, (Rat) P84079, (Rat) P84082, (Mouse) Q8BSL7, (Mouse) P61205, (Rat) P61206, (Rat) P61751, (Mouse) P61750, (Mouse) P84084, (Rat) P84083, (Mouse) P62331, (Rat) P62332
Entrez Gene ID: (Dog) 100856517, (Human) 375, (Dog) 486562, (Human) 377, (Dog) 607073, (Human) 378, (Dog) 475206, (Human) 381, (Human) 382, (Dog) 490679, (Chinese hamster) 100757864, (Mouse) 11840, (Rat) 64310, (Rat) 79119, (Mouse) 11841, (Mouse) 11842, (Chinese hamster) 100766631, (Rat) 140940, (Chinese hamster) 100769922, (Rat) 79120, (Mouse) 11843, (Mouse) 11844, (Chinese hamster) 100759676, (Rat) 79117, (Mouse) 11845, (Chinese hamster) 100774654, (Rat) 79121