OriGene

ARG1 Monoclonal Antibody (OTI4E6), TrueMAB™

Product Image Gallery

ARG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human liver tissue within the normal limits using anti-ARG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502413)

ARG1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARG1 (RC204649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG1. Positive lysates LY424951 (100 µg) and LC424951 (20 µg) can be purchased separately from OriGene.

ARG1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC204649 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARG1 antibody (TA502413), and then analyzed by flow cytometry.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Western Blot (WB)

1:1000-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4E6

Immunogen

Full length human recombinant protein of human ARG1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: ARG1

UniProt ID: (Human) P05089

Entrez Gene ID: (Human) 383

Molecular Function: hydrolase