OriGene

ARHGDIA Monoclonal Antibody (OTI1F2), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (20) RhoGDI antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

ARHGDIA Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (RC200902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHG. Positive lysates LY401371 (100 µg) and LC401371 (20 µg) can be purchased separately from OriGene.

ARHGDIA Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGDIA antibody (TA501089), and then analyzed by flow cytometry.

ARHGDIA Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in ARHGDIA knockout (KO) 293T cell line using Anti-ARHGDIA monoclonal antibody (Product # TA501089). {KO}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

Western Blot (WB)

1:200-1:1000

Product Specifications

Species

Dog, Human, Rat

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1F2

Immunogen

Full length human recombinant protein of human ARHGDIA produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: arhgdia {ECO:0000312|RGD:1359547}; epididymis secretory sperm binding protein Li 47e; GDP-dissociation inhibitor, aplysia RAS-related 1; MGC117248; Rho GDI 1; rho GDI 1 {ECO:0000250|UniProtKB:P19803}; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; rho GDP-dissociation inhibitor 1 {ECO:0000250|UniProtKB:P19803}; Rho-GDI alpha; rho-GDI alpha {ECO:0000250|UniProtKB:P19803}

Gene Aliases: ARHGDIA; Bles01; GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

UniProt ID: (Human) P52565, (Rat) Q5XI73

Entrez Gene ID: (Human) 396, (Dog) 475924, (Rat) 360678

Molecular Function: G-protein modulator enzyme modulator signaling molecule