Bethyl Laboratories

ARHGDIB/D4-GDI/RhoGDI2 Polyclonal Antibody

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ARHGDIB/D4-GDI/RhoGDI2 Antibody in Western Blot (WB) — **Detection of human ARHGDIB/D4-GDI by western blot.** *Samples:* Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. *Antibody:* Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody A304-815A-M (lot A304-815A-M-1) used for WB at 1:1000. *Detection:* Chemiluminescence with an exposure time of 10 seconds.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:1,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 50 and 100

Conjugate

Unconjugated

Form

liquid

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; Rho GDI 2; Rho GDP dissociation inhibitor (GDI) beta; Rho GDP-dissociation inhibitor 2; Rho-GDI beta; RhoGDI2

Gene Aliases: ARHGDIB; D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2

UniProt ID: (Human) P52566

Entrez Gene ID: (Human) 397

Molecular Function: G-protein modulator enzyme modulator signaling molecule