OriGene

ARHGEF2 Monoclonal Antibody (OTI5F2), TrueMAB™

Product Image Gallery

ARHGEF2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGEF2 (RC204546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHG. Positive lysates LY417793 (100 µg) and LC417793 (20 µg) can be purchased separately from OriGene.

ARHGEF2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of permeabilized Hela cells, using anti-ARHGEF2 antibody (TA809320), (Red), compared to negative control (PBS), (Blue). (1:100)

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI5F2

Immunogen

Full length human recombinant protein of human ARHGEF2 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

GEF-H1 activates Rho GTPases by promoting the exchange of GDP and GTP. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. GEF-H1 may be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; LFP40; Microtubule-regulated Rho-GEF; NEDMHM; Proliferating cell nucleolar antigen p40; Rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2

Gene Aliases: ARHGEF2; GEF; GEF-H1; GEFH1; KIAA0651; LFP40; P40

UniProt ID: (Human) Q92974

Entrez Gene ID: (Human) 9181

Molecular Function: G-protein modulator enzyme modulator guanyl-nucleotide exchange factor