|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the amino terminus of human ARP3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with endogenous levels of total ARP2 protein.
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enterohemorrhagic E. coli requires N-WASP for efficient type III translocation but not for EspFU-mediated actin pedestal formation.
PA5-17288 was used in western blot to investigate the role of N-WASP in type III translocation in enterohemorrhagic Escherichia coli
|Vingadassalom D,Campellone KG,Brady MJ,Skehan B,Battle SE,Robbins D,Kapoor A,Hecht G,Snapper SB,Leong JM||PLoS pathogens (6:null)||2010|