Western blot detection of ARTS in extracts of 293T cells transfected with ARTS plasmid. Samples were separated on SDS-PAGE and probed using an ARTS monoclonal antibody (Product # MA1-24693) at a dilution of 1:4000.The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues N(247) I Q D N R V H C C L Y F I S P F G H G(266) corresponding to residues 247-266 of human ARTS|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24693 detects ARTS from human samples. MA1-24693 is expected to cross react with mouse (100% conserved) due to sequence homology.
MA1-24693 has been successfully used in immunocytochemistry, ELISA, and Western blot procedures.
The MA1-24693 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues N(247) I Q D N R V H C C L Y F I S P F G H G(266) corresponding to residues 247-266 of human ARTS.
This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein encoded by this gene is thought to be part of a complex involved in cytokinesis. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.