Invitrogen

ATF7 Recombinant Rabbit Monoclonal Antibody (JJ2-072)

View all (11) ATF7 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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ATF7 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of ATF7 in HepG2 cells using a ATF7 Monoclonal antibody (Product # MA5-32440) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ATF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ATF7 of paraffin-embedded Mouse skeletal muscle tissue using a ATF7 Monoclonal antibody (Product #MA5-32440). Counter stained with hematoxylin.

ATF7 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of ATF7 in Jurkat cells using a ATF7 Monoclonal Antibody (Product # MA5-32440) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32440

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JJ2-072
Immunogen	Synthetic peptide within Human ATF7 aa 1-50 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809718

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Isoform 4/ATF-A0acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter. Isoform 5/ATF-4acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating transcription factor 7; cAMP-dependent transcription factor ATF-7; Cyclic AMP-dependent transcription factor ATF-7; MGC57182; Transcription factor ATF-A

Gene Aliases: 1110012F10Rik; 9430065F09Rik; AI549878; ATF7; ATFA; C130020M04Rik

UniProt ID: (Human) P17544

Entrez Gene ID: (Human) 11016, (Rat) 315333, (Mouse) 223922

Molecular Function: basic leucine zipper transcription factor