|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen|
|Purification||Ammonium sulfate precipitation|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10-50 µg/ml|
|Western Blot (WB)||10-50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glycerol (1:1) may be added for additional stability.
Reconstitute with 500 ul of distilled water.
Centrifuge to remove any insoluble material.
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.