Immunofluorescent analysis of ATG4D in untreated U-251 cells (left) and U-251 cells treated with Chloroquine (50 uM,16h) (right) using an ATG4D monoclonal antibody (Product # MA5-18110) at a dilution of 1:25, followed by detection using a Dylight 488-conjugated secondary antibody at a dilution of 1:400. Nuclei were stained with Dapi (blue).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||ATG4D recombinant protein|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.