|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: mouse wildtype ES cell lysate.
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
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Protein Aliases: APG5; APG5-LIKE; APG5L; apoptosis specific protein; Apoptosis-specific protein; ATG5 autophagy related 5 homolog; autophagy 5-like protein; Autophagy protein 5; autophagy related 5; autophagy-related 5; hAPG5
Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; BOS_9664; C88337; hAPG5; Paddy; zgc:100934
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