Immunofluorescent analysis of ATGL in 3T3-L1 cells using an ATGL monoclonal antibody (Product # MA5-14990) (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
It is not recommended to aliquot this antibody.
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.
MA5-14990 was used in immunoprecipitation to study the interaction of FSP27 with adipose triglyceride lipase and its role in regulating adipocyte lipolysis and insulin signaling
|Grahn TH,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V||The Journal of biological chemistry (289:12029)||2014|