Invitrogen

ATIC Monoclonal Antibody (F38 P7 H9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (19) ATIC antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ATIC Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of ATIC expression in colon carcinoma using Product # MA1-086.

ATIC Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extract (30 µg) of Hep G2 (Lane 1) HCT 116 (Lane 2) K-562 (Lane 3), COLO 205 (Lane 4), NTERA-2 (Lane 5) and HT -29 (Lane 6). The blots were probed with Anti-ATIC Mouse Monoclonal Antibody (Product # MA1-086, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 65 kDa band corresponding to ATIC was observed across cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

ATIC Antibody in Western Blot, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, IHC (P), IP) — Figure 2 The target antigen of XC154 mAb was oncogenic ATIC. ( A ) Preparative 10% SDS-PAGE to isolate XC154 antigen and in-gel digestion for the mass spectrometric protein identification. The protein band containing XC154 antigen, which was confirmed by Western blotting, was excised (indicated by the red arrow) and in-gel digested. Proteins identified by mass spectrometric analysis were listed in Table 1 . ( B ) The validation of XC154 antigen as ATIC. HepG2 cells were transfected with si-ATIC and their cell lysates were analyzed by Western blotting with XC154 mAb. beta-Actin was used as an internal control. ( C ) Immunoprecipitation analysis for the verification of XC154 antigen as ATIC. Red arrows indicate XC154 Ag or ATIC. ( D ) The expression of ATIC in liver tissues of H- ras12V -Tg mice. Blots were probed with commercial anti-ATIC antibody. ( E ) Immunohistochemical analysis of ATIC in liver tissues of HCC model mice. Liver tissues from wild type control mice (Non-Tg: WT) were also stained. NT: non-tumor, T: tumor region, H- ras12V -Tg ( n = 6), Non-Tg ( n = 3), HBx-Tg-nonT: HBx-transgenic mouse without tumor ( n = 5), HBx-Tg-ST: HBx-transgenic mouse with small tumor ( n = 4), HBx-Tg-LT: HBx-transgenic mouse with large tumor ( n = 7). Representative images were shown (All staining images were shown in Supplementary Figure S2 ). ( F ) ATIC in exosomes purified from hepatoma cell cultured media (HepG2 and Huh7 cells; 35 mug) analyzed by Western blotting. A well-known exo

ATIC Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK 293 cells were transfected with ATIC siRNA and loss of signal was observed in Western Blot using Anti- ATIC monoclonal Antibody (Product # MA1-086). {KD}

Product Details

MA1-086

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	View 1 publication 1 publication
Flow Cytometry (Flow)	1 µg / 10^6 cells	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Fruit fly, Human, Mouse, Rat, Xenopus
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	F38 P7 H9
Immunogen	Synthetic peptide, conjugated to Ovalbumin, corresponding to residues A(583) H T N L R L F H H(592) of human ATIC. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2062489

Product Specific Information

MA1-086 detects the ATIC in human, rat, Xenopus, Drosophila, and mouse cells.

MA1-086 has been successfully used in Western blot and immunohistochemistry (parrafin) procedures. By Western blot, this antibody detects a ~64.6 kDa protein.

The MA1-086 immunogen is a synthetic peptide, conjugated to Ovalbumin, corresponding to residues A(583) H T N L R L F H H(592) of human ATIC..

Target Information

ATIC is a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591-amino acid protein that is 81% identical to the chicken sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cyclic Peptide Mimotopes for the Detection of Serum Anti-ATIC Autoantibody Biomarker in Hepato-Cellular Carcinoma.

International journal of molecular sciences

Heo CK,Hwang HM,Lim WH,Lee HJ,Yoo JS,Lim KJ,Cho EW

MA1-086 was used in Western Blotting to indicate that anti-ATIC autoantibody can be a potential HCC-associated serum biomarker and suggests that autoantibody biomarker's efficiency can be improved by using antigenic mimicry to native antigens present in vivo.

Sat Dec 19 00:00:00 EST 2020

Cyclic Peptide Mimotopes for the Detection of Serum Anti-ATIC Autoantibody Biomarker in Hepato-Cellular Carcinoma.

International journal of molecular sciences

Heo CK,Hwang HM,Lim WH,Lee HJ,Yoo JS,Lim KJ,Cho EW

Sat Dec 19 00:00:00 EST 2020

Cyclic Peptide Mimotopes for the Detection of Serum Anti-ATIC Autoantibody Biomarker in Hepato-Cellular Carcinoma.

International journal of molecular sciences

Heo CK,Hwang HM,Lim WH,Lee HJ,Yoo JS,Lim KJ,Cho EW

Sat Dec 19 00:00:00 EST 2020

Cyclic Peptide Mimotopes for the Detection of Serum Anti-ATIC Autoantibody Biomarker in Hepato-Cellular Carcinoma.

International journal of molecular sciences

Heo CK,Hwang HM,Lim WH,Lee HJ,Yoo JS,Lim KJ,Cho EW

Sat Dec 19 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase; AICAR formyltransferase; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR transformylase; AICAR transformylase/inosine monophosphate cyclohydrolase; AICARFT/IMPCHASE; ATIC; Bifunctional purine biosynthesis protein ATIC; Bifunctional purine biosynthesis protein ATIC, N-terminally processed; bifunctional purine biosynthesis protein PURH; epididymis secretory sperm binding protein Li 70p; IMP cyclohydrolase; IMP synthase; Inosine 5'-monophosphate cyclohydrolase; Inosinicase; Phosphoribosylaminoimidazolecarboxamide formyltransferase; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Gene Aliases: 2610509C24Rik; AA536954; AICAR; AICARFT; ATIC; AW212393; HEL-S-70p; IMPCHASE; OK/SW-cl.86; PURH; XELAEV_18044849mg

UniProt ID: (Human) P31939, (Rat) O35567, (Mouse) Q8BPF0

Entrez Gene ID: (Human) 471, (Rat) 81643, (Mouse) 108147, (Xenopus) 735175

Function(s)

brainstem development nucleobase-containing compound metabolic process 'de novo' IMP biosynthetic process nucleoside metabolic process purine ribonucleoside monophosphate biosynthetic process response to inorganic substance cerebellum development cerebral cortex development organ regeneration dihydrofolate metabolic process tetrahydrofolate biosynthetic process cell-cell adhesion cobalamin metabolic process ribonucleotide metabolic process purine nucleotide biosynthetic process metabolic process

Process(es)

IMP cyclohydrolase activity phosphoribosylaminoimidazolecarboxamide formyltransferase activity protein homodimerization activity cadherin binding involved in cell-cell adhesion catalytic activity transferase activity hydrolase activity

ATIC Monoclonal Antibody (F38 P7 H9)

Product Details

MA1-086

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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