Immunohistochemical analysis of ATM in formalin-fixed, paraffin-embedded human kidney tissue using an ATM monoclonal antibody (Product # MA1-23152) at a dilution of 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23152 detects ATM in human, rat, mouse and monkey samples.
MA1-23152 has been successfully used in ChIP Assay, ELISA, FACS, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and Western blot procedures. Predicted molecular weight of MA1-23152 is ~350kDa.
MA1-23152 immunogen corresponds to recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
ATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.