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Invitrogen
ATP Citrate lyase Recombinant Superclonal Antibody (8HCLC)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
ATP Citrate lyase Antibody (711766) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), NCI-H1975 (Lane 3), PC-3 (Lane 4), SK-OV-3 (Lane 5), A-431 (Lane 6), COS-7 (Lane 7), Jurkat (Lane 8), F9 (Lane 9) and tissue extracts of Mouse Lung (Lane 10). The blots were probed with Anti-ATP Citrate lyase Recombinant Rabbit Polyclonal Antibody (Product # 711766, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 ... View More
Product Details
711766
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
8HCLC
Immunogen
Protein corresponding to human ATP Citrate Lyase [aa1-aa425]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716907
Product Specific Information
This antibody predicted to react with Monkey, Sheep, Cat
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme
Gene Aliases: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92
Entrez Gene ID: (Human) 47, (Mouse) 104112
ATP citrate synthase activity
protein binding
ATP binding
metal ion binding
cofactor binding
nucleotide binding
catalytic activity
succinate-CoA ligase (ADP-forming) activity
transferase activity
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
transferase
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
acetyl-CoA metabolic process
lipid metabolic process
metabolic process
cellular carbohydrate metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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