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Bovine pulmonary artery endothelial cells: Mitochondria were labeled using an anti-OxPhos Complex V subunit a, mouse IgG<sub>2b</sub> monoclonal antibody (459240) prelabeled with the Zenon® Alexa Fluor® 555 Mouse IgG<sub>2b</sub> Labeling Kit (Cat. no. Z25205). Tubulin was detected with an anti–a-tubulin mouse IgG<sub>2b</sub> monoclonal antibody prelabeled with the Zenon® Alexa Fluor® 488 Mouse IgG<sub>2b</sub> Labeling Kit (Cat. no. Z25202), and The nucleus was stained with DAPI (Cat. no. D1306, D3571, D21490).
|Tested species reactivity||Bovine, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat, Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Bovine Complex V|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml per 10^6 cells|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
Use Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells for positive controls.
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamics of enhanced mitochondrial respiration in female compared with male rat cerebral arteries.
459240 was used in western blot to test if mitochondrial energetics of large cerebral arteries are sex dependent.
|Rutkai I,Dutta S,Katakam PV,Busija DW||American journal of physiology. Heart and circulatory physiology (309:H1490)||2015|
Sustained mitochondrial functioning in cerebral arteries after transient ischemic stress in the rat: a potential target for therapies.
459240 was used in western blot to test if mitochondrial function in the cerebral vasculature is maintained after transient middle cerebral artery occlusion in rats
|Rutkai I,Katakam PV,Dutta S,Busija DW||American journal of physiology. Heart and circulatory physiology (307:H958)||2014|
α-Synuclein is localized to mitochondria-associated ER membranes.
459240 was used in western blot to demonstrate that wildtype α-synuclein localizes mitochondria-associated endoplasmic reticulum membranes and that mutated α-synuclein behaves differently.
|Guardia-Laguarta C,Area-Gomez E,Rüb C,Liu Y,Magrané J,Becker D,Voos W,Schon EA,Przedborski S||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:249)||2014|
Influence of pregnancy in mid-to-late gestation on circulating metabolites, visceral organ mass, and abundance of proteins relating to energy metabolism in mature beef cows.
459240 was used in western blot to investigate the effect of pregnancy on BW change, carcass traits, visceral organ mass, and circulating serum metabolites in cows.
|Wood KM,Awda BJ,Fitzsimmons C,Miller SP,McBride BW,Swanson KC||Journal of animal science (91:5775)||2013|
Effects of SRC and STAT3 upon gap junctional, intercellular communication in lung cancer lines.
459240 was used in western blot to determine the contribution of the SRC/STAT3 axis to gap junctional communication suppression.
|Geletu M,Guy S,Raptis L||Anticancer research (33:4401)||2013|
Effect of moderate dietary restriction on visceral organ weight, hepatic oxygen consumption, and metabolic proteins associated with energy balance in mature pregnant beef cows.
459240 was used in western blot to study the effect of dietary restriction on the abundance of selected proteins regulating cellular energy metabolism in cows.
|Wood KM,Awda BJ,Fitzsimmons C,Miller SP,McBride BW,Swanson KC||Journal of animal science (91:4245)||2013|
Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart.
459240 was used in western blot to test if mitochondrial quality control mechanisms contribute to the development of cardiac dysfunction.
|Hoshino A,Mita Y,Okawa Y,Ariyoshi M,Iwai-Kanai E,Ueyama T,Ikeda K,Ogata T,Matoba S||Nature communications (4:null)||2013|
alpha subunit; ATP synthase; ATP synthase alpha chain; ATP synthase subunit alpha; ATP5A; ATP5AL2; ATPM; cardiac muscle; H+ transporting; hATP1; iso; isoform 1; mitochondrial; mitochondrial F1 complex; MOM2; OMR; ORM